DR. MOHAMED (MOH) SALEM
Office: Jones Hall 337
Mail: Box 60
My research interests are focused on using the state-of-the-art "genomics" technologies and experiments to study and characterize various physiological processes in non-model species. I use high throughput omics and bioinformatics approaches to study gene networks/pathways that regulate different physiological traits at genomics, transcriptomics, post-transcriptomics and proteomics levels. My projects include but are not limited to utilization of genomic approaches to examine and enhance fish production traits with particular interest in muscle growth and quality.
Next generation sequencing
Salem, M., Leeds, T. D. Rexroad, C. E., 3rd, Yao, J. (2012) RNA-seq indentifies genetic markers for fast growth in rainbow trout, PLoS One, 7(5): e36264.
Salem M: Next generation sequencing and functional analysis in rainbow trout. In: Functional Genomics in Aquaculture. Edited by Saroglia M, Liu Z: Wiley Blackwell; (2012).
Salem, M., Rexroad, C. E., 3rd, Thorgaard, G.H., Yao, J. (2010) Characterization of the rainbow trout transcriptome using Sanger and 454-Pyrosequencing approaches,BMC Genomics, 11:564.
Sanchez, C., Smith, T., Wiedmann, R., Vallejo, R., Salem, M., Yao J., Rexroad, C. E., 3rd. (2009). Single Nucleotide Polymorphism discovery in Rainbow Trout by deep sequencing of a reduced representation library, BMC Genomics, 10:559.
Microarray chip technology
Salem, M., Kenney, P. B., Rexroad, C. E., 3rd, Yao, J. (2006). Microarray gene expression analysis in atrophying rainbow trout muscle: a unique nonmammalian muscle degradation model, Physiol Genomics,28(1): 33-45.
Salem, M., Kenney, P.B., Rexroad, C. E., 3rd, Yao J. (2008). Development of a 37K high-density oligonucleotide microarray: A new tool for functional genome research in rainbow trout, J Fish Biol, 72:2187-2206.
Salem, M., Silverstein, J., Rexroad, C. E., 3rd, Yao, J. (2007). Effect of starvation on global gene expression and proteolysis in rainbow trout (Oncorhynchus mykiss), BMC Genomics, 8: 328.
Goravanahally, M. P., Salem, M., Yao, J., Inskeep, K., Flores, J.A.(2009). Identification of differential gene expression during transition of bovine corpus luteum from early to mid–phase and their potential role in acquisition of luteolytic sensitivity to prostaglandin f2 alpha., Biol Reprod,80(5):980-988.
Bettegowda, A., Patel, O.V., Lee, K. B., Park, K. E., Salem, M., Yao, J., Ireland, J. J., Smith, G. W. (2008). Identification of novel bovine cumulus cell molecular markers predictive of oocyte competence: functional and diagnostic implications, Biol Reprod, 79: 301–309.
Salem M, Xiao, C., Womack, J., Rexroad, C. E. 3rd, Yao, J.(2010). MicroRNA repertoire for functional genome research in rainbow trout (Oncorhynchus mykiss),Marine Biotech,12(4):410-29.
Ramachandra, R. K., Salem, M., Gahr, S., Rexroad, C. E., 3rd, Yao, J. (2008). Cloning and characterization of microRNAs from rainbow trout (Oncorhynchus mykiss): their expression during early embryonic development, BMC Dev Biol, 8: 41.
Tripuran, S., Xiao, C., Salem, M., Yao, J. (2010). Cloning and Expression Profiling of Fetal Ovary-Expressed microRNAs in Cattle, Anim Reprod Sci, 120: 16-22.
Proteomics and enzyme assays
Salem, M., Kenney, P.B., Rexroad, C. E. 3rd, Yao, J. (2010). Proteomic signature of muscle atrophy in rainbow trout, J Proteomics,73: 778-789.
Salem, M., Kenney, P., Killefer, J., Nath, J. (2004). Isolation and in vitro characterization of the calpains from rainbow trout (Oncorhynchus mykiss) muscle and their role in texture development, J. Muscle foods,15: 245-255.
Salem, M., Kenney, P.B., Rexroad, C. E., 3rd, Yao, J. (2006). Molecular characterization of muscle atrophy and proteolysis associated with spawning in rainbow trout, Comp Biochem Physiol D Genomics Proteomics, 1(2): 227-237.
Salem, M., Levesque, H., Moon, T. W., Rexroad, C. E., 3rd, Yao, J. (2006). Anabolic effects of feeding beta2-adrenergic agonists on rainbow trout muscle proteases and proteins. Comp Biochem Physiol A Mol Integr Physiol,144(2): 145-54.
Gene cloning and pathway characterization
Salem, M., Yao, J., Rexroad, C. E., 3rd, Kenney, P. B., Semmens, K., Killefer, J., Nath, J. (2005). Characterization of calpastatin gene in fish: its potential role in muscle growth and fillet quality, Comp Biochem Physiol B Biochem Mol Biol, 141(4): 488-97.
Salem, M., Nath, J., Rexroad, C. E., 3rd, Killefer, J., Yao, J. (2005). Identification and molecular characterization of the rainbow trout calpains (Capn1 and Capn2): their expression in muscle wasting during starvation, Comp Biochem Physiol B Biochem Mol Biol, 140(1): 63-71.
Wang,J., Salem, M., Kenney, P.B., Rexroad, C. E., 3rd, Yao, J. (2010). Molecular characterization of the MuRF genes in rainbow trout: potential role in muscle degradation, Comp Biochem Physiol, 158(3):208-15.
Salem, M., Nath, J., Killefer, J. (2004). Cloning of the calpain regulatory subunit cDNA from fish reveals a divergent domain-V, Anim Biotechnol, 15(2): 145-57.
Salem, M., Rexroad, C. E., 3rd, Yao, J. (2005). Identification of a novel gill-specific calpain from rainbow trout (Oncorhynchus mykiss), Fish Physiol Biochem, 32: 1-6.