Dr. Anthony L. Farone

Professor

Dr. Anthony  L. Farone
615-898-5343
Room 2083, Science Building (SCI)
MTSU Box 60, Murfreesboro, TN 37132

Degree Information

  • Ph.D., Miami University, Oxford, OH (1992)
  • M.S., Miami University, Oxford, OH (1988)
  • B.S., Pennsylvania State University (1984)

Areas of Expertise

  • Immunology
  • Microbiology
  • Immunomodulators

Biography

I am interested in the interaction of host immune cells called macrophages with intracellular pathogens. I am particularly interested in the interaction of macrophages with organisms known as "amoeba-resistant bacteria" . Because many of these organisms are unculturable by traditional methods, many of these microbes have remained undiscovered yet may play a role in upto 50% of pneumonias of unknown cause. The laboratory's research objective is to isolate and identify novel bacteria from amoeb...

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I am interested in the interaction of host immune cells called macrophages with intracellular pathogens. I am particularly interested in the interaction of macrophages with organisms known as "amoeba-resistant bacteria" . Because many of these organisms are unculturable by traditional methods, many of these microbes have remained undiscovered yet may play a role in upto 50% of pneumonias of unknown cause. The laboratory's research objective is to isolate and identify novel bacteria from amoeba and to characterize their interaction with both amoeba and human macrophage cell lines utilizing techniques such as DNA sequencing, real-time quantitative PCR, and cellular staining and microscopy, including brightfield, fluorescent, confocal, and transmission electron microscopy techniques.

My interest in disease-causing microbes has lead to collaboration with members of the Anthropology, Agriscience, Math, and Chemistry departments. I am studying possible pathogens in the runoff from the aerobic decomposition of animals with Anthropology and Agriscience. I am also collaborating with members of the Chemistry and Mathematics departments to use molecular modeling to characterize important amino acid residues in a nucleoside hydrolase active site of E. coli. Our Math, Chemistry, and Biology Biodynamics Group has currently begun work to utilize high level computational methods to model bacterial chemotaxis on medical devices.

Grants

  • MTSU Clean Energy Grant. 2011-2017. Co-PI. Bioremediation of Barbital Compounds for Eco-Friendly Composting ($32,000). Co-PIs: M. Farone (Biology) and P. Kline (Chemistry).
  • NSF Innovation-Corps Grant, Biopanel, co-PIs: Brock Arivett and Elliot Altman ($50,000)
  • NSF I-Corps Grant, Salomon's House co-PIs Matt Wright and Elliot Altman ($50,000)
  • NSF GK-12 Grant International Supplement. 2013-2016. PI. TRIAD: Reforming Graduate Education through Teaching, Research, and Industry Applications to Deepen Scientific Understanding. Co-PIs: M. Farone (MTSU Biology), K. Sadler with Universidad de Chile, Santiago and University of Puerto Rico ($75,000).
  • NSF GK-12 Grant. 2009-2016. PI. TRIAD: Reforming Graduate Education through Teaching, Research, and Industry Applications to Deepen Scientific Understanding ($3,000,000). Co-PIs: M. Farone (MTSU Biology), K. Sadler (MTSU Biology Education) and E. Myles (TSU Biology).

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Publications

  • Mehari YT, Arivett BA, Farone AL, Gunderson JH, Farone MB. 2016. Draft genome sequences of two novel amoeba-resistant intranuclear bacteria, “Candidatus Berkiella cookevillensis” and “Candidatus Berkiella aquae.” Genome Announc 4: pii: e01732-15. doi: 10.1128/genomeA.01732-15. PubMed PMID: 26893424; PubMed Central PMCID: PMC4759071.
  • Mehari YT, Hayes BJ, Redding, KS*, Mariappan PV, Gunderson JH, Farone AL, Farone MB. 2016. Description ...
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  • Mehari YT, Arivett BA, Farone AL, Gunderson JH, Farone MB. 2016. Draft genome sequences of two novel amoeba-resistant intranuclear bacteria, “Candidatus Berkiella cookevillensis” and “Candidatus Berkiella aquae.” Genome Announc 4: pii: e01732-15. doi: 10.1128/genomeA.01732-15. PubMed PMID: 26893424; PubMed Central PMCID: PMC4759071.
  • Mehari YT, Hayes BJ, Redding, KS*, Mariappan PV, Gunderson JH, Farone AL, Farone MB. 2016. Description of “Candidatus Berkiella aquae”and “Candidatus Berkiella cookevillensis,” two intranuclear bacteria of freshwater amoebae. Int J Syst Evol Microbiol. 66:536-541. doi: 10.1099/ijsem.0.0007500.1099/ijsem.0.000750.
  • Park, H, Vick, E, Gao, Y, He, C, Almosnid, N, Farone, M, Farone, A. 2016. Cis and trans-gnetin H isolated from Paeonia suffruticosa exerts anti-inflammatory effects in human THP-1 cells. Submission to J Ethnopharmacology. 189:202–9. doi:10.1016/j.jep.2016.05.042
  • Gao, Y., Huang, R., Gong, Y., Park, H. S., Wen, Q., Almosnid, N., Uma, D., Chippada-Venkata, U. D., Hosain, N., Vick, E., Farone, A., E. Altman. 2015. The antidiabetic compound 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione, isolated from Averrhoa carambola L., demonstrates significant antitumor potential against human breast cancer cells. Oncotarget. 6(27):24304-24319. doi:  10.18632/oncotarget.4475
  • Eric J. Vick, Hyo S. Park, Krista A. Huff, Kenneth M. Brooks, Anthony L. Farone, Mary B. Farone. 2014. Gardnerella vaginalis triggers NLRP3 inflammasome recruitment in THP-1 monocytes. J Reproductive Immunol. 106:67-75.
  • Pellizzaro, A., Farone, A. Farone, M., Mushi, R. Aguinaga, M., and D. Erenso.2012. Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient. Biomed Optics Express. 3:2190-2199.
  • Jackson, J., Farone, A., and M. Farone. 2011. Bacterial enteropathogens associated with diarrhea in a rural population of Haiti [ID 23426]. Res Rep Trop Med. 2:129-133.
  • Farone, A., Farone, M., Kline, P., and Z. Sinkala. 2010. A practical approach for computing the active site of the ribonucleoside hydrolase of E.coli encoded by rihC. Adv Exp Med Biol. 680:437-443.
  • Farone, A., Farone, M., Khaliq, A., Kline, P., Quinn, T., and Z. Sinkala, 2009. Identifying the active site of ribonucleoside hydrolase of E. coli encoded by RihC. Proceedings of the 2009 International Conference on Bioinformatics & Computational Biology: BIOCOMP2009 Vol. I, Editors: H.R. Arabnia and M. Q. Yang, CSREA Press, USA, pp 216-218.
  • Ward, R.L., McNeal, M.M., Farone, M.B., and A.L. Farone. 2007. "The Reoviridae" In: The Mouse in Biomedical Research. (A. Smith, ed.) 2nd Edition. Elsevier Scientific Publishing, Inc., New York. pp. 235-268.
  • Berk, S.G., Gunderson, J.H., Newsome A.L., Farone, A.L., Skolasinski, W.S., Hayes, B.J., Redding, K.S., Uddin, N., William, E.L., Reid, A., Ventrice, J.A., Skimmyhorn, J. and Farone, M. B. 2006. Occurrence of infected amoebae in cooling towers environments compared with natural aquatic environments: implications for emerging diseases. Environ. Sci. Technology 40:7440-7444.
  • Farone, A. L., O'Donnell, S.M., Brooks, C. S., Young, K. M., Pierce, J., Wetzel, D., Dermody, T. S. and Farone, M. B. 2006. Reovirus serotype-dependent inflammatory cytokine repsonses and replication patterns in a human monocyte cell line. Viral Immunol. 19:546-557
  • Farone, A. L., Farone, M. B., McNeal, M., Ward, R. Reoviridae and Epizootic Diarrhea of Infant Mice. 2006. In: The Mouse in Biomedical Research, 2nd Edition, J.G. Fox, C. Newcomer, A. Smith, S. Barthold, F. Quimby, M. Davisson, Eds. Academic Press, NY.
  • Hunt, C., Gillani, N., Farone, A., Rezaei, M. Kline, P. 2005. Kinetic isotope effects of nucleoside hydrolase from Escherichia coli. Biochimica et Biophysica Acta 1751, 140-149.
  • Farone, M., and Farone, A. 2005. Detecting mold spores in school buildings: An exercise in biodiversity. Am. Biol. Teach. 67: 401-410.

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Awards

  • 1996-2012 Official recognition every year by a graduating MTSU senior as "Someone Who Made a Difference"
  • 2009-2010 MTSU Excellence in Presentations
  • 2008-2009 MTSU Million Dollar Grantsmanship Award
  • 2006-2007 MTSU Research Mentor Award