BIOL 4450/6450 Molecular Genetics. Four credits. Six hours lecture/laboratory. Offered both Fall and Spring Semesters.
Prerequisites: BIOL 2230 Microbiology, BIOL 2231 Microbiology Lab, BIOL 3250 Genetics, BIOL 3251 Genetics Lab, CHEM 1110 General Chemistry I, CHEM 1111 General Chemistry I Lab, CHEM 1120 General Chemistry II, CHEM 1121 General Chemistry II Lab.
Basic techniques of microbial genetics and gene manipulation with emphasis on the application of molecular genetics in basic and applied research.
This course covers both classical and molecular genetics and utilizes Escherichia coli as a model system. Experiments include, phenotyping and genotyping, isolation and characterization of mutants, mapping of genes using high frequency of recombination (Hfr) strains and bacteriophage P1, determining gene order by three factor crosses, the lac operon and the use of lacZ as a biological marker, bacteriophage lambda biology and the use of lambda in molecular genetics, cloning genes using recombinant DNA technology including the polymerase chain reaction (PCR).