Interdisciplinary PhD in Molecular Biosciences

Dr. Paul C. Kline - Courses

CHEM 4500/6500 Biochemistry I Undergraduate/graduate split-level course Three credits. Three hours lecture Offered Fall semester

Prerequisite: CHEM 3020 Organic Chemistry II

Chemical and physical properties of amino acids, proteins, carbohydrates, lipids, and nucleic acids. Chemical basis of enzyme catalysis.

This course is the first semester of a two-semester sequence designed to introduce the student to the chemistry of living organisms. The course is organized into three main sections. The first is a review of organic chemistry, acid-base properties, and thermodynamics along with the properties of water. The second and largest section covers the structure of the four basic types of biomolecules: amino acids/proteins, carbohydrates, nucleotides, and lipids. Included in this section are the three-dimensional structure of proteins and the structure of biological membranes. The third section covers enzymatic catalysis and mechanisms along with enzyme kinetics and biochemical signaling.

CHEM 4510/6500 Biochemistry II Undergraduate/graduate split-level course Three credits. Three hours lecture Offered Spring semester

Prerequisite: CHEM 4500 Biochemistry I

Reactions of carbohydrate, lipid, amino acid and nucleotide metabolism. Emphasis on the chemistry of metabolic reactions. Transmission of genetic information.

This course is the second semester of an introductory biochemistry course. The first section of the class covers the metabolism of carbohydrates. The citric acid cycle is included in this section. A number of topics starting with the electron transport system and the synthesis of ATP are introduced in the second section. This is followed by the metabolism of lipids including  oxidation and the synthesis of steroids. The metabolism of amino acids are also studied. Finally the control and regulation of metabolism are included. The third section covers the synthesis and degradation of nucleotides and nucleic acids. The structure of nucleic acids and their replication are included in this section. The study of nucleic acids including their transcription and translation and the regulation of these processes complete the course.

CHEM 4530/6530 Biochemical Techniques Undergraduate/graduate split-level course Two credits. Six hours laboratory Offered Spring semester

Prerequisite/corequisite: CHEM 4500 Biochemistry I

Laboratory in biochemical techniques with emphasis on protein purification, enzyme kinetics, carbohydrate and lipid analysis and manipulation of DNA

Biochemical Techniques is a laboratory-based course designed to provide the student experience with the basic laboratory procedures used to isolate, characterize, and analyze proteins, carbohydrates, lipids, and nucleic acids. Techniques covered in the class include ion exchange, centrifugation, mass spectrometry, gas chromatography, electrophoresis, size exclusion chromatography, Western blotting, and polymerase chain reaction (PCR). Emphasis is on gaining practical experience in the use of this techniques.