Dr. Paul C. Kline

Professor

Dr. Paul C. Kline
615-898-5466
Room 3079, Science Building (SCI)
MTSU Box X102, Murfreesboro, TN 37132

Degree Information

  • Ph.D., University of Notre Dame (1992)
  • M.S., Georgia Institute of Technology (1983)
  • B.S., Christian Brothers University (1979)
  • B.S., Christian Brothers University (1979)

Areas of Expertise

  • Bioorganic and Biophysical Chemistry
  • Mechanistic Enzymology and Transition State Analysis of Enzyme-Catalyzed Reactions
  • Compound purification, identification and synthesis

Publications

  • Cole, Ashley E.; Hani, Fatmah M.; Allen, Brian, W.; Kline, Paul C.; Altman, Elliot  Nonfunctional Missense Mutants in Two Well Characterized Cytosolic Enzymes Reveal Important Information About Protein Structure and Function, The Protein Journal, 2018, 37, 407-427. 
  • Bagsby, C.; Saha, A.; Goodin, G.; Siddiqi, S.; Farone, M.; Farone, A.; Kline, P.C. Stability of pentobarbital in soil. Journal of Environmental Science and Health B 2018, 53, 207-213.&n...
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  • Cole, Ashley E.; Hani, Fatmah M.; Allen, Brian, W.; Kline, Paul C.; Altman, Elliot  Nonfunctional Missense Mutants in Two Well Characterized Cytosolic Enzymes Reveal Important Information About Protein Structure and Function, The Protein Journal, 2018, 37, 407-427. 
  • Bagsby, C.; Saha, A.; Goodin, G.; Siddiqi, S.; Farone, M.; Farone, A.; Kline, P.C. Stability of pentobarbital in soil. Journal of Environmental Science and Health B 2018, 53, 207-213. 
  • Black, W.; Villareal, J.; Melton, T.; Kline, P. Identification of Disaccharides Using HPLC: An Undergraduate Experiment. Chem. Educator2015, 20, 9-12. Arivett, B.; Farone, M.; Masiragani, R.; Burden, A.; Judge, S.; Osinloye, A.; Minici, C.; Degano. M.; Robinson, Kline. P. Characterization of inosine-uridine nucleoside hydrolase (RihC) from Escherichia coli. Biochim. Biophys. Acta Proteins and Proteomics 2014, 1844, 656-662. 
  • McIntyre, E.; Melton, T.; Kline, P. Ion Exchange Chromatography of Proteins Using Microspin Columns:  An Undergraduate Biochemistry Laboratory. Chem. Educator 2012, 17, 1-2. Farone, A.; Farone, M.; Kline, P.; Quinn, T.; Sinkala, Z. A Practical Approach for Computing the Active Site of the Ribonucleoside Hydrolase of E. coli Encoded by rihC. In Advances in Computational Biology; Arabnia, H., Ed.; Advances in Experimental Medicine and Biology Series 680; Springer: New York, 2011; pp 437-443. 
  • Kline, P.C.; Zhao, H.Q.; Noll, B.C.; Serianni, A.S. 1-(b-D-Erythrofuranosyl)adenosine. Acta Crystallographica Section C Crystal Structure Communications 2010, C66, 215-218.
  • Merkler, David J.; Asser, Alexander S.; Baumgart, Laura E.; Carballo, Natalie; Carpenter, Sarah E.; Chew, Goeffrey, H.; Cosner, Casey C.; Dusi, Jodi; Galloway, Lamar C.; Lowe, Andrew B.; Lowe, Jr. Edward W.; King III, Lawrence; Kendig, Robert D.; Kline, Paul C.; Malka, Robert; Merkler, Kathleen A.; McIntyre, Neil R.; Romero, Mindy; Wilcox, Benjamin J.; Owen, Terence C. Substituted Hippurates and Hippurate Analogs as Substrates and Inhibitors of Peptidylglycine a-Hydroxylating Monooxygenase (PHM). Bioorganic and Medicinal Chemistry 2008, 16 10061-10074.
  • “Extraction of a Lipid from Nutmeg”, Tammy J. Melton and Paul C. Kline. Caché House Publishers, labPass Database,www.Cachehouse.com, 2007. 
  • “Spectral Analysis of Beef Extract”, Tammy J. Melton and Paul C. Kline. Caché House Publishers, labPass Database, www.Cachehouse.com,2007. 
  • “Identification of Disaccharides”, Tammy J. Melton and Paul C. Kline. Caché House Publishers, labPass Database, www.Cachehouse.com,2007.
  • Kline, P.C.; Noll, B.C.; Zhao, H.; Serianni, A.S. 1-( b -D-Erythrofuranosyl)cytidine (b-erythrocytidine). Acta Crystallographica Section C Crystal Structure Communications 2007, C63, 137-140.
  • Bates C.; Z. Kendrick Z.; McDonald, N; Kline, P. C. Transition State Analysis of Adenosine Nucleosidase from Yellow Lupin ( LupinuslLuteus). Phytochemistry 2006, 67, 5-12.
  • Hunt, C.; Gillani, N.; Farone, A.; Rezaei, M.; Kline, P.C. Kinetic Isotope Effects of Nucleoside Hydrolase from Escherichia coli. Biochim. Biophys. Acta Proteins and Proteomics 2005, 1751, 140-149.
  • Abusamhadneh, E.; McDonald, N. E.; Kline, P. C. Purification and characterization of adenosine nucleosidase form yellow lupin ( Lupinus luteus). Plant Sci. 2000, 153, 25-32.
  • Verdel, E. F.; Kline, P. C.; Wani, S.; Woods, A. E. Purification and partial characterization of haloperoxidase from fresh water algae Cladophora glomerata. Comp. Biochem. Physiol. B 2000, 125, 179-187.
  • Kline, P. C.; Razaei, M.; Lee, T. A. Determination of Kinetic Isotope Effects for Nucleoside Hydrolases using Gas Chromatography/Mass Spectrometry. Anal. Biochem. 1999, 275, 6-10.

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Awards

Grants

  • Technology Access Fee Grant, "Upgrade of Biochemistry Teaching Laboratories"; 2003, $170,000
  • American Chemical Society Petroleum Research Fund, "Transition State Analysis of Adenosine Nucleosidase" 1998-2000, $30,000.
  • P. C. Kline and A. Friedli, MTSU Technology Access Fees to purchase Gas Chromatographs for Undergraduate Organic and Biochemistry Laboratories. $40,000.

Research/Scholarly Activity

Directed Dissertations

  • Mansoureh Rezaei, "Transition State Analysis of Thymidine Phosphorylase from E. coli"; D.A. Dissertation 2001
  • Barbara J. Walton, "Selection, Modification, and Evaluation of Laboratory Experiments for a One-Semester Introductory Biochemistry Course" D.A. Dissertation 1998.
  • Nancy McDonald, "Characterization of Adenosine Nucleosidase from Lupinus Luteus" D.A. Dissertation 1998.

Theses

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Directed Dissertations

  • Mansoureh Rezaei, "Transition State Analysis of Thymidine Phosphorylase from E. coli"; D.A. Dissertation 2001
  • Barbara J. Walton, "Selection, Modification, and Evaluation of Laboratory Experiments for a One-Semester Introductory Biochemistry Course" D.A. Dissertation 1998.
  • Nancy McDonald, "Characterization of Adenosine Nucleosidase from Lupinus Luteus" D.A. Dissertation 1998.

Theses

  • Michael Kearney, "Kinetic Isotope Effects of Thymidine Phosphorylase" M.S. Thesis 1998.
  • X. Liu, "Characterization of Adenosine Nucleosidase from Yellow Lupin Seeds" M.S. Thesis 1998.
  • Fariborz Rohakhtar, "Investigation of the Structure of a Stable Complex Between Purine Nucleoside Phosphorylase and Hypoxanthine," M.S. 1997.
  • E. Abusamhadneh, "Isolation and Characterization of Adenosine Nucleosidase from Yellow Lupin Seeds" M.S. Thesis 1997.

Grants

  • Technology Access Fee Grant, "Upgrade of Biochemistry Teaching Laboratories"; 2003, $170,000
  • American Chemical Society Petroleum Research Fund, "Transition State Analysis of Adenosine Nucleosidase" 1998-2000, $30,000.
  • P. C. Kline and A. Friedli, MTSU Technology Access Fees to purchase Gas Chromatographs for Undergraduate Organic and Biochemistry Laboratories. $40,000.

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